DOI: http://dx.doi.org/10.18203/2320-1770.ijrcog20210311

Role of autologous bone marrow derived stem cells and platelet rich plasma for endometrial regeneration and repair and ovarian rejuvenation

Manjula Anagani, Prabha Agrawal, Bollavaram Vikas Reddy, Prabhu Chandra Mishra

Abstract


Background: Stem cells are undifferentiated cells with a potential for self- renewal and differentiation into multiple mature cell types. PRP is blood plasma that has been enriched with platelets and multiple growth factors that can stimulate cellular processes and activate multi-potent stem cells to generate new, younger tissue and new blood vessels relevant to ovarian rejuvenation and endometrial regeneration.

Methods: This study was to evaluate the effectiveness of ABMDSC and PRP application in patients with thin endometrium refractory to treatment, premature ovarian failure, infertility and menopause. 37 symptomatic women between age group 18 years to 56 years were selected for ABMDSC and PRP instillation in endometrium and/ or ovaries. PRP was instilled in endometrium in 8 patients, in ovaries in 5 patients and in both endometrium and ovaries in 24 patients.

Results: There was significant improvement in endometrial thickness, along with improved blood flow to both endometrium and ovaries. FSH levels decreased and ovarian volume increased. There were three confirmed pregnancies with one delivery.  Menopausal symptoms decreased in 2 patients and spontaneous resumption of menses was seen in 1 patient of POF.

Conclusions: Stem cell therapy serves as a game changer with their unique properties, offering solutions for scores of women suffering from POF, poor oocyte quality, endometrial degeneration/ damage or menopausal symptoms.

 


Keywords


Bone marrow-derived stem cell, Platelet rich plasma, Premature ovarian failure, Thin endometrium

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References


Rejuvenation of Premature Ovarian Failure with Stem Cells (ROSE-1), University of Illinois at Chicago, 2016, Available at https://clinicaltrials.gov/ct2/show/NCT02696889. Accessed on 30 August 2020.

Herraiz S, Romeu M. Autologous stem cell ovarian transplantation to increase reproductive potential in patients who are poor responders. Fertn Stert. 2018;110(3):496-505.

Zhao Y, Chen S. Using mesenchymal stem cells to treat female infertility: an update on female reproductive diseases. Fertn Stert. 2019:9071720.

Łos MJ, Skubis A, Ghavami S. Stem cells and biomaterials for regenerative medicine. Stem Cells. 2019;2:5-16.

Dunlop CE, Telfer EE, Anderson RA. Ovarian germline stem cells. Stem Cell Res Therapy. 2014;5:98.

Johnson J, Canning J, Kaneko T, Pru JK, Tilly JL, Germline stem cells and follicular renewal in the postnatal mammalian ovary. Nature. 2004;428:145-50.

White YA, Woods DC, Takai Y, Ishihara O, Seki H, Tilly JL. Oocyte formation by mitotically active germ cells purified from ovaries of reproductive-age women. Nat Med. 2012;18:413-21.

Singh N, Mohanty S, Seth T, Shankar M, Dharmendra S, Bhaskaran S. Autologous stem cell transplantation in refractory Asherman’s syndrome: a novel cell based therapy. J Human Reproductive Sci. 2014;7(2):93-8.

Christianson M, Segars J. Unleashing the potential of stem cells to help poor responders American Society for Reproductive Medicine. 2018;110(3):410-11.

Abd-Allah SH, Shalaby SH, Pasha HF. Mechanistic action of mesenchymal stem cell injection in the treatment of chemically induced ovarian failure in rabbits, Cytotherapy. 2013;15(1):64-75.

Badawy A, Sobh MA, Ahdy M, Abdelhafez MS. Bone marrow mesenchymal stem cell repair of cyclophosphamide-induced ovarian insufficiency in a mouse model. Int J Women's Health. 2017;9:441-7.

Kawamura K. Hippo signaling disruption and Akt stimulation of ovarian follicles for infertility treatment. Proc Natl Acad Sci. 2013;110(43):17474-9.

Ferrari M, Zia S, Valbonesi M, Henriquet F, Venere G, Spagnolo S, et al. A new technique for hemodilution, preparation of autologous platelet-rich plasma and intraoperative blood salvage in cardiac surgery, Int J Artif Organs. 1987;10(1):47-50.

Arshdeep, Kumaran MS. Platelet-rich plasma in dermatology: boon or a bane? Indian J Dermatol Venereol Leprol. 2014;80:5-14.

Chang Y, Li J, Chen Y, Wei L, Yang X, Shi Y, et al. Autologous platelet-rich plasma promotes endometrial growth and improves pregnancy outcome during in vitro fertilization. Int J Clin Exp Med. 2015;8:1286-90.

Nazari L, Salehpour S, Hoseini S, Zadehmodarres S, Ajori L. Effects of autologous platelet-rich plasma on implantation and pregnancy in repeated implantation failure: A pilot study. Int J Reprod Biomed. 2016;14(10):625-8.

Zadehmodarres S, Salehpour S, Saharkhiz N, Nazari L. Treatment of thin endometrium with autologous platelet-rich plasma: a pilot study. JBRA Assist Reprod. 2017;21(1):54-6.

Reversing menopause: new technique claims to ‘rejuvenate’ ovaries. Available at https:// globalnews.ca/news/2846089/reversing-menopause-new-technique-claims-to-rejuvenate-ovaries/. Accessed on 29 August 2020.

Sills ES. Platelet-rich plasma (PRP) infusions and biomarkers of ovarian rejuvenation and aging, inovium ovarian rejuvenation trials. Available at https://clinicaltrials.gov/ct2/show/NCT03178695. Accessed on 29 August 2020.

Zhang S, Li P, Yuan Z, Tan J. Platelet-rich plasma improves therapeutic effects of menstrual blood-derived stromal cells in rat model of intrauterine adhesion. Stem Cell Res Therapy. 2019;10:1.