DOI: https://dx.doi.org/10.18203/2320-1770.ijrcog20221917
Published: 2022-07-27

Effect of gold nanocomposites treatment on male reproductive function under conditions of experimental hyperglycemia

Oksana N. Kaleynikova, Svetlana I. Ukrainska, Valentine A. Sribna, Vasyl Y. Velykiy, Alena A. Vinogradova-Anyk, Katerina V. Tarasova, Tatiana S. Lagodich, Igor M. Karvatskiy, Tetyana Y. Voznesenska, Taras V. Blashkiv

Abstract


Background: Given the urgency of the problem of clinical and experimental pathology of diabetes mellitus (DM) and the reproductive function disorders and their correction, the direction of research was determined in the following way: to establish the functional state of testicular cells and appendages under experimental hyperglycemia, and to assess future prospects for gold nanoparticles for clinical trials. The aims to evaluate the effect of five times treatment with gold nanocomposites (gold nanoparticles (AuNPs) in the polymer matrix D-g-PAA(PE) on male reproductive function under conditions of experimental hyperglycemia.

Methods: Experiments (two series) have been conducted on 120 (60 males and 60 females) Albino white laboratory mice (weighing 25-30 gm). Experimental hyperglycemia, a model of type 2 diabetes mellitus (DM2) was reproduced by administration of nicotinamide and streptozotocin (internally peritoneally). The treatment of substances was carried out in the following way: D-g-PAA (PE) (10.00 mg/kg), D-g-PAA (PE)/AuNPs (9.78 mg/kg) in the tail vein, in 0.3 ml, once a day, five times, 2 weeks after EG induction once a day, five times.

Results: it was established for the first time that there is disorder of male reproductive function under conditions of experimental hyperglycemia and five-fold treatment of gold nanocomposites (D-g-PAA(PE)/AuNPs, namely an increase in the number of abnormal sperm and a decrease in spermatids, as well as an increase in preimplantation mortality of embryos (compared to this value under experimental hyperglycemia).

Conclusions: Our new data suggest that treatment with such gold nanocomposites (gold nanoparticles in D-g-PAA (PE) polymer matrix) are not critical for therapeutic use (in photodynamic chemotherapy), even in hyperglycemia when there is diabetes.


Keywords


Polymer matrix, Gold nanoparticles, Male reproductive function, Testis, Epididymis, Experimental hyperglycemia

Full Text:

PDF

References


Balasubramanian S, Jittiwat J, Manikandan J, Ong Ch, Yu L, Ong W. Biodistribution of gold nanoparticles and gene expression changes in the liver and spleen after intravenous administration in rats. Biomaterials. 2010;31(8):2034-42.

Li W, Wang F, Liu Zh, Wang Yu, Wang J, Sun F. Gold nanoparticles elevate plasma testosterone levels in male mice without affecting fertility. Small. 2013;9(9-10):1708-14.

Brohi R, Wang L, Talpur H, Wu D, Khan F, Bhattarai D et al. Toxicity of Nanoparticles on the Reproductive System in Animal Models: A Review. Front Pharmacol. 2017;8:606.

Omar A, Samir K. Does repeated gold-nanoparticles administration affect pars distalis hormonal and folliculo-stellate cells in adult male albino rats? Folia Histochem Cytobiol. 2021;59(2):95-107.

Fazan S, De Vasconcelos C, Valença M, Nessler R, Moore K. Diabetic peripheral neuropathies: a morphometric overview. Int J Morphol 2010;28:51-64.

Awad S, Al-Mawali A, Al-Lawati J, Morsi M, Critchley J, Abu-Raddad L. Forecasting the type 2 diabetes mellitus epidemic and the role of key risk factors in Oman up to 2050. Mathematical modelling analyses. 2021;12(7):1162-74.

Ding G, Liu Y, Liu M, Pan J, Guo M, Shen J et al. The effects of diabetes on male fertility and epigenetic regulation during spermatogenesis. Asian J Androl. 2015;17(6):948-53.

Maresch C, Stute D, Alves M, Oliveira P, de Kretser D, Linn T. Diabetes-induced hyperglycemia impairs male reproductive function: a systematic review. Human Reproduction Update. 2018;1;24(1):86-105.

Maresch C, Stute D, Fleming T, Lin J, Hammes H, Linn T. Hyperglycemia induces spermatogenic disruption via major pathways of diabetes pathogenesis. Scientific Rep. 2019;9:13074.

Tian Y, Song W, Xu D, Chen X, Li X, Zhao Y. Autophagy Induced by ROS Aggravates Testis Oxidative Damage in Diabetes via Breaking the Feedforward Loop Linking p62 and Nrf2. Hindawi Oxidative Med Cellular Longevity. 2020;7156579:9.

Gofur A, Witjoro A, Arifah S, Atho’illah M, Annisa Y, Lestari S. Black soybean tempeh and purple sweet potato improve sperm quality in streptozotocin-induced diabetic rats. Veterinary World. 2020;2231-916.

Kutsevol N, Bezugla T, Bezuglyi M, Rawiso M. Branched dextran-graft-polyacrylamide copolymers as perspective materials for nanotechnology. Macromol Symp. 2012;317-318(1):82-90.

Bezuglyi M, Kutsevol N, Rawiso M, Bezugla T. Water-soluble branched copolymers dextran-polyacrylamide and their anionic derivates as matrices for metal nanoparticles in-situ synthesis. Chemik. 2012;8(66):862-7.

Kutsevol N, Bezuglyi M, Rawiso M, Bezugla T. Star-like destran-graft-polyacrylamide-co-polyacrylic acid. Copolymers. Macromol Symp. 2014;335:12-6.

Yeshchenko O, Kutsevol N, Naumenko A. Light-induced heating of gold nanoparticles in colloidal solution: dependence on detuning from surface plasmon resonance. Plasmonics. 2016;11(1):345-50.

Chumachenko V, Naumenko A, Yeshchenko O, Kutsevol N, Bondarchuk I. Synthesis, morphology and optical properties of Au/Cds hybride nanocomposites stabilized by branched polymer matrices. J Nanomater 2016.

Kutsevol N, Chumachenko V, Harahuts Yu, Marinin A. Aging process of gold nanoparticles synthesized in situ in aqueous solutions of polyacrylamides. In: Mukbanianym OV, Abadie MJ, Tatrishvili T (eds) Chemical engineering of polymers Production of functional and flexible materials, part 2, chapter 10. Apple Academic Press, Florida. 2017:119-29.

Telegeev G, Kutsevol N, Chumachenko V, Naumenko A, Telegeeva P, Filipchenko S et al. Dextran-graft-polyacrylamide as matrices for creation of anticancer nanocomposite. Int J Polym Sci. 2017;9:1-9.

Kutsevol N, Naumenko A, Harahuts Yu, Chumachenko V, Shton I, Shishko E et al. New hybrid composites for photodynamic therapy: synthesis, characterization and biological study. Appl Nanosci. 2019;9(5):881-8.

Reagan-Shaw S, Nihal M, Ahmad N. Dose translation from animal to human studies revisited. FASEBJ. 2008;22(3):659-61.

Al-Awar A, Kupai K, Veszelka M, Szűcs G, Attieh Z, Murlasits Z et al. Experimental Diabetes Mellitus in Different Animal Models. J Diabetes Res. 2016;9051426.

Kleinert M, Clemmensen Ch, Hofmann S, Moore M, Renner S, Woods S et al. Animal models of obesity and diabetes mellitus. Nature Reviews. Endocrinology. 2018;14:141-62.

Kottaisamy C, Raj D, Kumar V, Sankaran U. Experimental animal models for diabetes and its related complications-a review. Lab Animal Res. 2021;37:23.

Darenskay M, Kolesnikova L. Kolesnikov S. Oxidative Stress: Pathogenetic Role in Diabetes Mellitus and Its Complications and Therapeutic Approaches to Correction. Bull Exp Biol Med. 2021;171:179-89.

Matkovics B, Kotorman M, Varga I, Hai D, Varga C. Oxidative stress in experimental diabetes induced by streptozotocin. Acta Physiol Hung. 1997-1998;85(1):29-38.

Styskal J, Van Remmen H, Richardson A, Salmon A. Oxidative stress and diabetes: what can we learn about insulin resistance from antioxidant mutant mouse models? Free Radic Biol Med. 2012;1;52(1):46-58.

Tiwari B, Pandey K., Abidi A, Rizvi S. Markers of Oxidative Stress during Diabetes Mellitus. J Biomarkers. 2013;378790.

Soldatov A, Gostyukhina O, Golovina I. Soldatov A. Functional states of the antioxidant enzymatic complex of tissues of Mytilus galloprovincialis Lam. under conditions of oxidative stress. Zh Evol Biokhim Fiziol. 2014;50(3):183-9.

Sribna V, Kaleinikova O, Kuziv Y, Vinogradova Anyk A, Karvatskiy I, Voznesenskaya T et al. Effect of one-time dextran-polyacrylamide polymer matrixes treatment on female reproductive function. Int J Reprod Contracept Obstet Gynecol. 2020;9(6):2317-22.

Blashkiv T, Sribna V, Kutsevol N, Kaleinikova O, Kuziv Y, Voznesenskaya T. Effect of silver nanocomposites treatment on female reproductive function. World J Pharmaceutical Med Res. 2020;6(4):124-32.

Kaleynikova O, Ukrainska S, Sribna V, Kutsevol N, Kuziv Y, Vinogradova-Anyk A et al. Effect of gold nanocomposite treatment on male reproductive function. Appl Nanosci. 2021.

Kaleynikova O, Ukrainska S, Blashkiv T. Effect of gold nanocomposites treatment on male reproductive function under conditions of experimental chronic kidney disease. Applied Nanosci. 2022.

Mamina V, Sheiko L. The assessment of functional state of testis and ovaries of rats after chrome hexavalent exposure. Problems Reproduct. 2017;1:25-8.