DOI: http://dx.doi.org/10.18203/2320-1770.ijrcog20212145

Influence of maternal age on intracytoplasmic sperm injection outcome and global deoxyribonucleic acid methylation in women undergoing intracytoplasmic sperm injection cycle

Mohammed M. Laqqan, Maged M. Yassin

Abstract


Background: Intracytoplasmic sperm injection needs sufficient oocytes of high quality in order to increase the rate of fertilization and pregnancy. This study was designed to investigate the influence of maternal age on the ICSI outcomes in women undergoing to first ICSI cycle and to evaluate the influence of maternal age on global DNA methylation.

Methods: A total of 242 females were included in this study with a mean age of 30.5±7.3 years. The participants were divided into three groups depending on women's age≤25, N=70; 26-35, N=102 and>35, N=70). The genomic DNA was isolated from the blood samples, then the global DNA methylation was evaluated using ELISA.

Results: A significant reduction has been found in the level of anti-Müllerian hormone (AMH), total number of the collected oocyte, mature oocytes, fertilized oocytes and number of embryos transferred in the older females compared to the younger group (p<0.001). While a significant increase has been found in global DNA methylation level in the older females compared to the younger group (p<0.001). A positive significant correlation has been found between global DNA methylation level and maternal age (p<0.001). In contrast, a negative significant correlation has been shown between AMH level, mature oocytes and maternal age (p<0.001).

Conclusions: Maternal age has a significant influence on the number of mature oocytes, number of embryos transferred and global DNA methylation. The pregnancy chance is more in the age group less than 35 years.


Keywords


Age, Female infertility, Global DNA methylation, ICSI outcomes

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References


Hakimi O, Cameron LC. Effect of exercise on ovulation: a systematic review. Sports Med. 2017;47(8):1555-67.

Kumar N, Singh AK. Trends of male factor infertility, an important cause of infertility: a review of literature. J Hum Reprod Sci. 2015;8(4):191-6.

Capalbo A, Hoffmann ER, Cimadomo D, Maria Ubaldi F, Rienzi L. Human female meiosis revised: new insights into the mechanisms of chromosome segregation and aneuploidies from advanced genomics and time-lapse imaging. Hum Reprod Update. 2017;23(6):706-22.

Khalaf Y, El‐Toukhy T, Coomarasamy A, Kamal A, Bolton V, Braude P. Selective single blastocyst transfer reduces the multiple pregnancy rate and increases pregnancy rates: a pre‐and postintervention study. BJOG Int J Obstet Gynaecol. 2008;115:385-90.

Rehman R, Mahmood H, Syed F, Syed H, Gul H. Intracytoplasmic sperm injection and advanced maternal age: Success or treatment failure. Pakistan J Pharmaceut Sci. 2019;32(4):1495.

Mills M, Rindfuss RR, McDonald P, TeVelde E, ESHRE Reproduction and Society Task Force. Why do people postpone parenthood? Reasons and social policy incentives. Hum Reprod Update. 2011;17(6):848-60.

Karim SH. The effect of maternal age on the outcomes of in vitro fertilization Sulaimani region. Mustansiriya Med J. 2016;15(1):70-4.

Nelson SM, Lawlor DA. Predicting live birth, preterm delivery, and low birth weight in infants born from in vitro fertilisation: a prospective study of 144,018 treatment cycles PLoS Med. 2011;8:1000386.

Schmidt L, Sobotka T, Bentzen JG, Nyboe Andersen A, ESHRE Reproduction and Society Task Force. Demographic and medical consequences of the postponement of parenthood. Hum Reprod Update. 2012;18(1):29-43.

Grøndahl ML, Christiansen SL, Kesmodel US, Agerholm IE, Lemmen JG, Lundstrøm P, et al. Effect of women’s age on embryo morphology, cleavage rate and competence-a multicenter cohort study PLoS One. 2017;12(4):0172456.

Kohda T, Ishino F. Embryo manipulation via assisted reproductive technology and epigenetic asymmetry in mammalian early development. Biolog Sci. 2013;368(1609):20120353.

Brenet F, Moh M, Funk P, Feierstein E, Viale AJ, Socci ND, Scandura JM. DNA methylation of the first exon is tightly linked to transcriptional silencing. PloS One. 2011;6:14524.

Schübeler D. Function and information content of DNA methylation. Nature. 2015;517:321-6.

Zaghlool SB, Al-Shafai M, Al Muftah WA, Kumar P, Falchi M, Suhre K. Association of DNA methylation with age, gender, and smoking in an Arab population. Clinical Epigenet. 2015;7(6):1-2.

Celik H, Kramer A, Challen GA. DNA methylation in normal and malignant hematopoiesis. Int J Hematol. 2016;103:617-26.

Hirasawa R, Chiba H, Kaneda M, Tajima S, Li E, Jaenisch R, Sasaki H. Maternal and zygotic Dnmt1 are necessary and sufficient for the maintenance of DNA methylation imprints during preimplantation development. Genes Dev. 2008;22(12):1607-16.

Li E, Zhang Y. DNA methylation in mammals. Cold Spring Harbor Perspect Biol. 2014;6:019133.

Heyn H, Vidal E, Ferreira HJ, Vizoso M, Sayols S, Gomez A, et al. Epigenomic analysis detects aberrant super-enhancer DNA methylation in human cancer. Genome Biol. 2016;17:1-6.

Marshall KL, Wang J, Ji T, Rivera RM. The effects of biological aging on global DNA methylation, histone modification, and epigenetic modifiers in the mouse germinal vesicle stage oocyte. Animal Reproduct. 2018;15(4):1253-67.

Menken J, Trussell J, Larsen U. Age and infertility. Science. 1986;233(3):1389-94.

LaMarca A, Spada E, Sighinolfi G, Argento C, Tirelli A, Giulini S, et al. Age-specific nomogram for the decline in antral follicle count throughout the reproductive period. Fertil Steril. 2011;95(2):684-8.

Balasch J. Ageing and infertility: an overview. Gynecol Endocrinol. 2010;26(12):855-60.

Wyndham N, Figueira PG, Patrizio P. A persistent misperception: assisted reproductive technology can reverse the “aged biological clock”. Fertil Steril. 2012;97(5):1044-7.

Hornstein MD. State of the ART: assisted reproductive technologies in the United States. Reproduct Sci. 2016;23:1630-3.

Tan TY, Lau MS, Loh SF, Tan HH. Female ageing and reproductive outcome in assisted reproduction cycles. Singapore Med J. 2014;55(6):305-9.

Chan CH, Chan TH, Peterson BD, Lampic C, Tam MY. Intentions and attitudes towards parenthood and fertility awareness among Chinese university students in Hong Kong: a comparison with Western samples. Human Reproduct. 2015;30(2):364-72.

Dunson DB, Baird DD, Colombo B. Increased infertility with age in men and women. Obstet Gynecol. 2004;103(1):51-6.

Silber SJ, Nagy Z, Devroey P, Camus M, VanSteirteghem AC. The effect of female age and ovarian reserve on pregnancy rate in male infertility: treatment of azoospermia with sperm retrieval and intracytoplasmic sperm injection. Human Reproduct. 1997;12(12):2693-700.

Çiray HN, Ulug U, Tosun S, Erden HF, Bahceci M. Outcome of 1114 ICSI and embryo transfer cycles of women 40 years of age and over. Reproduct Biomed Online. 2006;13(4):516-22.

Dew JE, Don RA, Hughes GJ, Johnson TC, Steigrad SJ. The influence of advanced age on the outcome of assisted reproduction. J Assist Reproduct Genet. 1998;15(4):210-4.

Ron-El R, Raziel A, Strassburger D, Schachter M, Kasterstein E, Friedler S. Outcome of assisted reproductive technology in women over the age of 41. Fertil Steril. 2000;74:471-5.

Moon SY, Kim SH, Jung BJ, Jee BC, Suh CS, Lee JY. Influence of female age on pregnancy outcome in in vitro fertilization and embryo transfer patients undergoing intracytoplasmic sperm injection. J Obstet Gynaecol Res. 2000;26(1):49-54.

Kenny LC, Lavender T, McNamee R, O’Neill SM, Mills T, Khashan AS. Advanced maternal age and adverse pregnancy outcome: evidence from a large contemporary cohort. PloS One. 2013;8(2):56583.

Bellver J, Ayllón Y, Ferrando M, Melo M, Goyri E, Pellicer A, et al. Female obesity impairs in vitro fertilization outcome without affecting embryo quality. Fertil Steril. 2010;93(2):447-54.

Choi HW, Park YS, Lee SH, Lim CK, Seo JT, Yang KM. Effects of maternal age on embryo quality and pregnancy outcomes using testicular sperm with intracytoplasmic sperm injection. Clinic Experiment Reproduct Med. 2016;43(4):221.

Ahmed M, Shareef O, Adam I, Rayis D. Maternal age and intracytoplasmic sperm injection outcome in infertile couples at Khartoum, Sudan. F1000Res. 2015;4:1339.